Product Info Summary
SKU: | A06145-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-MMP20 Antibody Picoband™
SKU/Catalog Number
A06145-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MMP20 Antibody Picoband™ catalog # A09039-2. Tested in WB, ELISA applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MMP20 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06145-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human MMP20 recombinant protein (Position: Y124-K405). Human MMP20 shares 91.5% amino acid (aa) sequence identity with mouse MMP20.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06145-2 is reactive to MMP20 in Human
Applications
A06145-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.387kDa
Background of MMP20
Matrix metalloproteinase-20 (MMP-20) also known as enamel metalloproteinase or enamelysin is an enzyme that in humans is encoded by the MMP20 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MMP20 using anti-MMP20 antibody (A06145-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP20 antigen affinity purified polyclonal antibody (Catalog # A06145-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP20 at approximately 54 kDa. The expected band size for MMP20 is at 54 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MMP20 (Source: Uniprot.org, NCBI)
Gene Name
MMP20
Full Name
Matrix metalloproteinase-20
Weight
54.387kDa
Superfamily
peptidase M10A family
Alternative Names
AI2A2; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.22; EC 3.4.24.35; Enamel metalloproteinase; Enamelysin; matrix metallopeptidase 20; matrix metalloproteinase 20 (enamelysin); matrix metalloproteinase-20; MMP-20 MMP20 AI2A2, MMP-20 matrix metallopeptidase 20 matrix metalloproteinase-20|enamel metalloproteinase|matrix metalloproteinase 20 (enamelysin)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP20, check out the MMP20 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP20 Antibody Picoband™ (A06145-2)
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Customer Reviews
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